亚投彩票

肿瘤模型之PDX与CDX

RENYUANHUAZHONGLIUMOXINGDEYIYIZAINA?

WEN:SHENMESHIZHONGLIUYIZHIMOXING?

DA:RENDEZHONGLIUYIZHIDAOXIAOSHUTINEIGOUJIANDERENYUANHUAZHONGLIUMOXING

WEN:ZHEIZHONGMOXINGYIYIZAINA?

DA:SHAIYAO,WEIRENMINFUWU。(QINGWEIWEIDADEXIAOSHUZHIJING!)

WEN:WEISHAYAOGOUJIANZHONGLIUMOXING,BUNENGTIAOGUOZHIJIESHANGLINCHUANG?BUYEKEYISHAIYAOMA?

DA:NG,HAOZHUYI,YAOBUNIXIANLAIHAOBUHAO?

…….


SHENMESHICDXZHONGLIUMOXING

CDX(RENYUANZHONGLIUXIBAOXIYIZHONGYIZHI,cell derived  xenograft)MOXINGZHUYAOSHIJIANGZHONGLIUXIBAOXIYIZHIDAOLUOSHUHUOZHENSGXIAOSHUTINEIERGOUJIANDEZHONGLIUMOXING.ERYOUYUZHONGLIUXIBAOXISHIJINGGUORENGONGCHUNHUAYIJIDUODAIPEIYANGDEXIBAOXI,SUOYIQIGOUJIANDECDXMOXINGSHIWUFABAOCHIZHONGLIUZUZHIDEYIZHIXINGDE,TONGSHI,YEYOUYUQIJINGGUOLEDUOCIDECHUANDAI(JISHICICHUANDAI),SUOYIQISHENGWUXUETEZHENGBIANHUAMINGXIAN,CONGERSHIDEQISHENGWUXUETEXINGYIJIYAOXIAOPINGJIAJIEGUOYULINCHUANGXIANGSIDUJIDI.


SHENMESHIPDXZHONGLIUMOXING

PDX(RENYUANZHONGLIUZUZHILAIYUANYIZHILIUMOXING ,patientl derived  xenograft)MOXINGSHIJIANGZHONGLIUZUZHIYIZUZHIDEXINGSHIYIZHIZHINSGXIAOSHUTINEI,CONGERHENHAODEBAOCHILEZHONGLIUDEYIZHIXING,TONGSHI,PDXMOXINGSHIJIANGZHONGLIUZUZHIZHIJIEYIZHIDAONSGXIAOSHUTINEI,BINGMEIYOUJINGGUORENHERENGONGPEIYANG,SUOYIQISHENGWUXUETEXINGBAOCHIDEGENGJIAWANZHENG,CONGERYULINCHUANGXIANGSIDUGENGGAO,SHIXIANJIEDUANZUIYOUXIUDEZHONGLIUDONGWUMOXING.


HUASHUOZAOZAI20SHIJI90NIANDAIZAOQI,MEIGUOGUOLIAIZHENGYANJIUSUO(National Cancer Institute, NCI)JIU YIJULAIYUANYU9ZHONGBUTONGLEIXINGZHONGLIU(NAO,JIECHANGAI,BAIXUEBING,FEI,HEISULIU、LUANCHAOAI、SHENAI、RUXIANAIHEQIANLIEXIANAI) DE60WEIAIZHENGHUANZHEZHONGLIUXIBAOXI,YINRULEYIZHONG”disease-oriented”DEYAOWUSHAIXUANCELVE,JIANDANLAISHUO,JIUSHIXIANYONGRENZHONGLIUXIBAOXIJINXINGGAOTONGLIANGTIWAIYAOWUSHAIXUAN,ZAIYONGCDXMOXINGJINXINGTINEIYANZHENG,YOUYUCDXMOXINGXIBAOXIRONGYIHUODE,YOUDALIANGYIFABIAODEWENXIANGUANYUQIJIYINZUXUE、XIBAOGONGNENGXUEJIYAOXIAOFANYINGDESHUJUKEGONGCANKAO,JIANMOCHENGBENDIDENGYOUSHI,ZAIDANGSHIKEYISHUOSHIFENGMIGEDASHIYANSHI。

DANSHI2016NIAN,NCIQUEJUEDINGCONGQIYAOSHAIXITONGZHONGRANGYIJINGSHIYONGLE25NIANDENCI60XIBAOXI“TUIXIU!

WEISHENMEHUIFASHENGZHEIZHONGZHUANBIAN?!

YUANLAI,YANJIUZHEZHUJIANFAXIANRENYUANZHONGLIUXIBAOXIJINGZHANGQITIWAIPEIYANGHOU, QIZHONGLIUXIBAOSHENGWUXUEXINGWEIJIJIYINPUBIAODASHUIPING、 ZHONGLIUYIZHIXINGDOUYUYUANSHIZHONGLIUZUZHICUNZAIJIAODADECHAYI, CONGERZAIYUCELINCHUANGYAOXIAOFANGMIANBUSHENLIXIANG。YOUYANJIUBIAOMING, JINGCIMOXINGJIANDINGSHAIXUANDEYAOWUJINYUE 1/3ZAIERQILINCHUANGSHIYANZHONGYANZHENGYOUXIAO。

BUGUO,ZHANGJIANGHOULANGTUIQIANLANG,TUIXIULECDX,JIUHUIYOUGENGYOUXIUDEZHONGLIUMOXINGJUEQI!

JIRANNENGSHIKEYANJIEYANYONGJISHINIANDECDXBEI“TUIXIU”,NEIJIUBIRANDAIBIAOXINSHENGLILIANGPDXQINGCHUYULAN,MUQIANGAIMOXINGBEIYANJIUZHEPUBIANRENKEDEYOUSHIZHUYAOBAOKUO:

1、移植所用标本直接来源于人体肿瘤组织,未经过体外培养,稳定地保留了肿瘤的遗传特性、组织学和表型特征,即肿瘤异质性;

RUXIATUSUOSHI,WEIGOUJIANDERENYUANJIEZHICHANGAIXIAOSHUYIZHIMOXING,TONGGUOBUTONGXINGTAIXUETEZHENGDEZUZHIRANSE,FAXIANZAIMOUXIEQINGKUANGXIA,XINXIANBINGZAOHECHUANDAIBINGZAOJUNBIAOXIANCHULIANGHAODEFENHUABIAOXING,MOUXIEYANGBENJUNCHUXIANTOUMINGXIBAO,NIANYEFENMIXIBAOHEXIBAOHUAISIQUYUDENG,TONGSHI,SHAOSHUZHONGLIUBIAOXIANCHUGAODUDEDUOXINGXING,BAOCHIWEIFENHUAZHUANGTAI(Fig2B)。

2.PDXKEYONGYUSHAIXUANHUALIAOYAOWUMINGANHUONAIYAOBIAOJIWU,QISHIYANJIEGUOJUYOUJIAOHAODELINCHUANGYUJIANXING

YIWEIAIPDXWEILI,TUABIAOSHIDUI cetuximabZHILIAOBUMINGANYUMINGANXIAOSHUZHONGEGFR DNA/mRNA/EGFRSHOUTIDANBAIKAOBEISHUCHAYI,FAXIANMINGANXIAOSHUZHONGKAOBEISHUPUBIANJIAOGAO,BINGQIETONGGUOZHONGLIUSHENGZHANGQUXIAN,IHC, FISHDUIBI,ZUIZHONGDECHU:DUI cetuximabMINGANDEXIAOSHUMOXING,QIEGFRBIAODAPUBIANSHANGDIAO。

CHUCIWAI,LINGYOUPDXZAIYIZHIGUOCHENGZHONGJIAOHAODIBAOLIULEZHONGLIUJIANZHIHEGANXIBAOCHENGFEN,SHIDEZHONGLIUDESHENGZHANGWEIHUANJINGGENGJIEJINSHIJIQINGKUANG,HAIKEWEIZHONGLIUYANGBENDEBAOCUNHECHUANDAITIGONGDALIANGBIAOBENDENG。

JIANMOSHIWEILESHENME?SHAIYAO!

ZHEIXIEHELIUXIAOSHUKEYIZUOWEIBINGRENDE“TISHEN”(avatar),DAITIBINGRENQUCESHIBUTONGYAOWUFANGANDEZHILIAOXIAOGUO,CONGZHONGSHAIXUANCHUZUIYOUXIAODEZHILIAOFANGAN,CONGERBIMIANYAOWUDUFUZUOYONGYIJIJINGJISHANGDELANGFEI,JUTILIUCHENGCANZHAOXIATU。

DANGRANLE,SUIRANPDXYOUSHIPODUO,DANJUXIANXINGYEBURONGHUSHI。

MUQIANPDXMOXINGYUANSHIZHONGLIUDEZHUYAOLAIYUANWEISHOUSHUQIECHU,JIANMONANDUGAOQIEBUNENGFANFUHUOQU,GOUJIANSHIJIANZHANGQIECHENGGONGLVBUWENDING,SUIZHECHUANDAICISHUZENGJIAZHONGLIUWEIHUANJINGYEHUIZHUJIANBEIXIAOSHUXIBAOWAIJIZHIQUDAI,YINCIDUIYUCHUANDAICISHUYOUYIDINGXIANZHI。ZHIDEYITIDESHI,FUHEZHONGLIUDEXIAOSHUJUNWEIMIANYIQUEXIANDEXIAOSHU,YINERGAIMOXINGYEWUFAYONGYUSHAIXUANMIANYIXIANGGUANYAOWU。

总的来说,两种模型各有千秋,某种程度上CDX可以被认为是传代次数太多已经难以追溯的PDX模型,经过体内外交替传代,原始肿瘤组织中只有最适应体外培养条件的单个克隆被保留下来,丧失了克隆异质性,从而难以对临床药效进行预测。

ERPDXZUIDADEYOUSHIYEZAIYUCI,BAOLIULEZHONGLIUYIZHIXING,GENGFUHELINCHUANGZHONGLIUTEZHENG。

标签:CDX肿瘤模型PDX与CDXPDX肿瘤模型肿瘤模型 下一篇:
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