亚投彩票

酒精液体饲料建立ALD大小鼠模型

ZAIXIFANGGUOJIA,JIUJINGZHONGDUSHIGANYINGHUADEZHUYAOBINGYIN。ZAIWOGUO,JIUJINGXINGGANSUNSHANG(ALD)DEFABINGLVYERIYIQUZENG。YINCI,DUIJIUJINGXINGGANSUNSHANGDELEJIEJIZHIYUBIANDEYOUWEIZHONGYAO。 MUQIAN,WEILEZHILIAOJIUJINGXINGGANBING,GUONEIWAIJIANLILEDUOZHONGDONGWUMOXINGLAIYANJIUJIUJINGXINGGANSUNSHANG。JIUJINGXINGGANSUNSHANGMOXINGKEFENWEIJIXINGYUMANXINGLIANGZHONG,BENWENZHUYAOFENXIJIZHONGTONGGUOYINSHIYOUDAODEMANXINGJIUJINGXINGGANSUNSHANGMOXING。

慢性酒精性肝损伤模型

酒精液体饲料模型

YOUZHUMINGJIUJINGYANJIULINGYUZHUANJIACharles S.LieberCHUANGLI,SHIYINGYONGZUIYOUJIU、ZUIGUANGFANDEDONGWUMOXING。

造模机制

ZAOQIDEMANXINGJIUJINGWEIYANGMOXING,RONGYIZAOCHENGDONGWUQUESHUI、YINGYANGBULIANG,QIEZHIJIESIWEIJIUJINGBINGBUNENGYINQIMINGXIANDEGANSUNSHANG。GAIMOXINGTONGGUOZAIYETISILIAODEJICHUTIANJIAGAONONGDUJIUJING,KEFUYINGYANGBULIANGDENGQUEDIAN,MONIRENLEIMANXINGYINJIUMOSHI,ZAOCHENGGANXIBAOZHIFANGBIANXING、ZHONGXINGLIXIBAOWEIZHUDEYANXIBAO。

造模方法

CAIYONGJIANKANGDEXIONGXINGWisterDASHUHUOSDDASHU,DASHUJINTOUWEIHANJIUJINGDEYETISILIAO,JIUJINGDESHERULIANGDADAOMEIRI12~18g/kg.

GAIYETISILIAODEPEIFANGHANFENMOZHUANGLAODANBAI、YUMIYOU、GANLANYOU、YOUXUANTANGGAN、MAIYATANG、JIUJING、WEISHENGSU、HUNHEYANLEI、JIAOCHACAIJIAO。QIZHONGJIUJINGJIYULIANGZHANZONGRELIANGDE40%ZUOYOU。HUOZHEZAISHIYONGJIUJINGYETISILIAODEJICHUSHANG,JIARUYIDINGLIANGDEBIZUO,KECUJINJIUJINGXINGGANSUNSHANGDEXINGCHENG,TIGAOMOXINGFUZHILV,SUODUANJIANMOZHOUQI。

TONGGUOZHEIZHONGWEIYANGFANGSHI4~12ZHOU,KEMOFANGRENLEIMANXINGYINJIUMOSHI,ZUIZHONGKEDAOZHIDASHUGANZANGQINGDUGANSUNSHANG、GANZHIFANGBIANXINGHEQINGWEIDEGANZANGYANZHENG。

优点:操作简易、费用低,成模率高。

缺点:肝脏没有出现纤维化,也没有规避动物厌恶酒精的天性。

NIAAA模型

GUOQUJIANLIDEYIXIEALDDONGWUMOXING,HUOZHEGANSUNSHANGQINGWEI,HUOZHENANYUCAOZUO,ERYOUNIAAAGANBINGYANJIUSHIJIANLIDEXINMOXING,JIMANXINGJIUJINGWEIYANGJIAJIXINGJIUJINGGUANWEIDEALDXIAOSHUMOXING(NIAAAMOXING),GENGJIEJINYURENLEIDEYINJIUFANGSHI,GANSUNSHANGGENGMINGXIAN。

造模机制

MONIRENLEIMANXINGYINJIUMOSHI,MANXINGJIUJINGWEIYANGJIAJIXINGJIUJINGGUANWEI,XIETONGZUOYONGDAOZHIXIAOSHUDEZHIFANGBIANXING、GANSUNSHANGJIYANZHENG。

造模方法

CAIYONGC57BL/6XIAOSHU,XIANJIYUXIAOSHU5TIANDEJIUJINGYETISILIAOSHIYINGQI,RANHOUCAIYONGHAN5%DEJIUJINGYETISILIAOWEIYANG10TIAN,ZAIDI11TIANSHANGWU7~9DIANDUIXIAOSHUJINXING1CIGAONONGDUJIUJINGGUANWEI,JILIANGWEI5 g/kgTIZHILIANG。

CHUCIZHIWAI,Gao-BingeMOXINGHAIBAOKUOJIZHONGGAIJINBANDEMANXINGALDMOXING,JIANGJIUJINGYETISILIAOWEIYANGYANZHANGZHI8~12ZHOU,ZUIZHONGJIYU1CIHUOZHEDIEJIADUOCIGAOJILIANGJIUJINGGUANWEI。

优点:操作简易、费用低、造模周期短、重复率高

缺点:短期喂养无病死率,无明显肝纤维化。

基因敲除模型

SUIZHEKEXUEJISHUDEFAZHAN,GUONEIWAIYUELAIYUEDUODEYANJIURENYUANCAIYONGJIYINQIAOCHUJISHUDENGLAIYANJIUJIUJINGXINGGANBINGDEJIZHI,RUHiroshikonoDENGYONGJIYIN(ICAM-1)QIAOCHUXIAOSHUJINXINGSHIYAN,BINGJIYUGAIXIAOSHUJIUJINGYETISILIAO,CONGERYANJIUICAM-1ZAIZAOQIJIUJINGXINGGANSUNSHANGXIAOSHUZHONGDEZUOYONG。

标签:液体饲料酒精肝模型饲料 上一篇: 下一篇:
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