亚投彩票

浅谈无菌小鼠饲料的营养标准

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研究表明,饲料经121℃,60分钟高压蒸汽灭菌后可达无菌要求,精氨酸、赖氨酸、异亮氨酸、酪氨酸损失在25%以下,维生素A、B1、B2、B6损失较多,一般成分和无机盐无明显变化,室温下隔离器内最多保存10天;饲料经50kGy60CO-γ射线辐照灭菌后可达无菌要求,粗蛋白、蛋氨酸明显减少,维生素A、E、B1、B6略微减少,其它营养成分无明显变化,且无菌小鼠饲料辐照灭菌的最低有效剂量为25kGy,最高耐受剂量为50kGy.

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由于无菌小鼠的肠道内没有微生物,其自身无法合成B族维生素及维生素K,所以无菌小鼠饲料中应尤其关注维生素的添加水平。有研究者利用辐照后的AIN93G饲料饲喂无菌KM小鼠导致小鼠体型瘦弱,出现明显的脊柱凸起,毛发粘染脏乱;而在AIN93G饲料基础上提高维生素水平,经高剂量辐照后(50kGy)饲喂无菌KM小鼠,其生长繁殖情况均正常,表明额外添加维生素能够满足繁殖阶段无菌小鼠的身体需求。

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标签:无菌动物饲料 上一篇:
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