亚投彩票

四氯化碳和胆碱缺乏饲料诱导的NASH模型对比

BIJIAOCHANGYONGDEJIASUNAFLDHENASHDONGWUMOXINGDEYOUDAOFANGFA,LIRUSILVHUATAN(CCl4)HEDANANSUANDANJIANQUEFA(MCD)YINSHI,YIJIDIERDAIDANJIANQUEFAYINSHI。

开发临床前NASH纤维化模型

1. SHIYONGSILVHUATANJINXINGYANZHENG、ZHIFANGBIANXINGHEXIBAOSIWANGDEDUSUYOUDAO

2. TONGGUOMCDSILIAOYOUDAODEGANZHIFANGBIANXING

3. ZENGQIANGNASHDEXINYIDAIDANJIANQUEFAYINSHI,MEIYOUZAOQIMOXINGZHONGGUANCHADAODEZHONGDUTIZHONGJIANQING

BENWENDUIBILEZHEIXIECHUANTONGXIANWEIHUAMOXING,TANTAOTAMENZAIXIANWEIHUAHENASHYAOWUKAIFAFANGMIANDEYOUSHIHEJUXIANXING。

四氯化碳诱导NASH模型

RONGJISILVHUATAN(CCl4)SHIYIZHONGDUSU,TONGGUOGANZANGXIBAOSESUP450DAIXIEWEISANLVJIAJIZIYOUJI(–CCl3)。ZHEIHUIYUXUDUOGANZANGBABIAOFASHENGFANYING,DADAODUOZHONGLEISINASHDEXIAOYING:

  • 线粒体活性氧簇引发的炎症
  • 脂代谢酶活性降低和高尔基氏体驱动的VLDL分泌受损导致的脂肪变性
  • 广泛的细胞损伤,导致细胞死亡和纤维化

ZHEIXIEYOUHAIXIAOYINGZAIMEIYOUFEIPANGHUOYIDAOSUDIKANGDEQINGKUANGXIAFASHENG,BINGQIESHEJIDALIANGYUZHIZHIZHUJIHEDAIXIEWUGUANDEXIBAOSIWANG。YINCI,ZHEIBINGBUNENGDAIBIAOZHENSHIDENAFLD。

CHULEQUEFADUIRENLEINAFLD/NASHDEZHENZHENGKEYIXINGWAI,GAIMOXINGDEZHUYAOQUEDIANSHIYINGXIANGFEIGANZANGQIGUANHEZUZHIDEQUANSHENDUXING,QIEYOUYUCCI4DUICAOZUORENYUANJUYOUQIANZAIHUAXUEWEIHAI,SUOYIZHINENGYOUXUNLIANYOUSUDEJISHURENYUANZHIXING,BIXUPEIBEISHIDANGDEFANGHUSHEBEIHEHUAXUEANQUANJICHUSHESHI。

MCD饲料诱导NASH模型

蛋氨酸和胆碱是肝线粒体β氧化和极低密度脂蛋白(VLDL)合成所需的关键营养素。喂食MCD饲料时,甘油三酸酯的输出受到抑制,从而迅速诱导肝脂肪变性和炎症。

HECCl4YIYANG,MCDSILIAOYINQIYIYONGXINGHEKUAISUYOUDAOERGUANGSHOUHUANYING。LIANGZHOUNEIXUEQINGALT/ASTWENJIANSHANGSHENG,BINGQIELINCHUANGNAFLDHUOXINGPINGFENZAITONGYISHIJIANDUANNEIJIEJINZUIDAZHI。XIANWEIHUAYEXUNSUFASHENG,ZAIDUANDUAN4ZHOUNEIJIUHENMINGXIAN,10ZHOUNEIDADAOZHONGDU。ZAIBUCHAOGUO16ZHOUDEGUOCHENGZHONG,XIANWEIHUASHIKENIDE,YINCIYINGZAIGENGZHANGDESHIJIANFANWEINEIYUNXINGMOXING,YIPINGJIAKEGAISHANNASHDEYAOWU。DANSHI,DONGWUXUANZEBICCl4MOXINGSHOUDAOGENGDUOXIANZHI,C57Bl/6XIAOSHUSHISHOUXUANDEYIGANXIAOSHUPINXI;DASHUHUIJINZHANWEIWENJIANDEZHIFANGBIANXING,DANZHIYOUQINGWEIYANZHENG,QIEMEIYOUXIANWEIHUA。

MCDMOXINGYOUXUDUOZHUANYIHESHIJIANFANGMIANDEQUEDIAN,ZUIMINGXIANDESHI,DONGWUJINGLILEKUAISUERYANZHONGDETIZHONGJIANQING(CONGWEISHIMCDSILIAOKAISHICHAOGUO40%),YIJIXUEQINGPUTAOTANG、GANYOUSANSUANZHIHEDANGUCHUNJIANGDI。TIZHONGJIANQINGYEZAOCHENGCAOZUORENYUANZAIDONGWUCHUZHIHEJIYAOFANGMIANDENANDU,ERDAIXIEBIAOXINGZEYURENLEIZHUANGKUANGZHIJIEXIANGFAN。

第二代胆碱缺乏饮食诱导NASH模型

JINGZHENGMING,DIERDAIDANJIANQUEFAYINSHIKEZAINIECHIDONGWUZHONGYOUDAONAFLD/NASH,ERBUHUICHUXIANMCDSILIAOSIWEISHIDEZHONGDUTIZHONGJIANQING。DANJIANQUEFAXINGANJISUAN(CDAA)YINSHIKEYIYONGJINGQUEDENGLIANGDEZUOXUANANJISUANDAITIDANBAIZHIHANLIANG,JIESHOUGAISILIAODEC57Bl/6XIAOSHUZAI3ZHOUNEICHUXIANZHIFANGBIANXINGHEYANZHENG,XIANWEIHUAFASHENGZAIDI6ZHOU,BINGZAIDI21ZHOUZUOYOUBIANWEIQINGDUZHIZHONGDU,ZHEIYESHIKENIDE。

ZHEIZHONGYINSHIYESHIHEWistarDASHUYOUDAONAFLD/NASH,BINGQIEYUXIAOSHUDEJIBINGFAZUOHEJINZHANSHIJIANFANWEIXIANGJIN。JINGUANRENHENIECHIDONGWUTIZHONGJIANQINGDOUBUSHIGAIMOXINGDEQUEDIAN,DANSHIGAIMOXINGZHONGBUCUNZAINAFLDZHIQIANDERENLEIDAIXIEBIAOXING(YIDAOSUDIKANG、GAOXUETANGZHENG、ZHIXUEZHENGDENG)。

CDAASILIAODERELIANGZHIFANGHANLIANGCONG10-20%ZENGJIAZHI60%(GAOZHIDANJIANQUEFASILIAO)HUIJIASUBINGJIAZHONGNASH,BINGXIANZHUJIANGDIXIANWEIHUADEZIFAKENIXING。ZAIC57Bl/6XIAOSHUZHONG,GAIMOXINGHAIJIANGZAI24ZHOUNEIFAZHANWEIGANYINGHUA、MENJINGMAIGAOYAHEGANGONGNENGSHUAIJIE,YINCIDUIYUGANZANGJIBINGYANJIUXIANGMUERYANJUYOUDUOZHONGYONGTU。RANER,ZAIDANJIANQUEFAYINSHIDEDIEDAIZHONG,MEIYOUFEIPANG、GAOXUETANGZHENGHEGAOGANYOUSANZHIXUEZHENGDENGDAIXIEZONGHEZHENGDETEZHENG。

结论

JINGDIANDECCl4GANZANGDUXINGMOXINGYIJIDANJIANQUEFAYINSHIYOUDAODEZHIFANGBIANXINGDEDUOCIDIEDAI,WEINASHHEGANXIANWEIHUATIGONGLEYITAOTEZHENGMINGQUEQIEGUANGFANKEYONGDENIECHIDONGWUMOXING。ZAIXUANZESILVHUATANHUODANJIANQUEFAYINSHIMOXINGSHI,YINGJIEHETAMENDEYOUDIAN,TONGSHIKAOLVQIZAIRENTITIAOJIANXIAKEYIXINGFANGMIANDEZHUDUOJUXIANXING。

本文作者:中美冠科生物

标签:胆碱缺乏饲料 上一篇: 下一篇:
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